Optimizing Reconstitution and Storage of Lyophilized Peptides: A Practical Guide

Optimizing Reconstitution and Storage of Lyophilized Peptides: A Practical Guide

Proper handling ensures peptide stability and experimental reproducibility. This guide covers best practices for reconstitution solvents, storage temperatures, and shelf-life extension.

Lyophilized peptides offer extended shelf life but require careful handling to maintain integrity. Based on our internal stability studies, we recommend the following protocols for researchers and formulators:

1. Reconstitution:

• Use sterile, apyrogenic water or buffer (e.g., 0.1% acetic acid for basic peptides) for initial dissolution.

• Avoid vortexing; gently swirl or roll the vial.

• Aliquot immediately after reconstitution to minimize freeze-thaw cycles.

  

2. Storage:

• Long-term (unreconstituted): Store at -20°C or below in a desiccated environment. Under these conditions, our peptides typically maintain specification for 36 months.

• Short-term (reconstituted): Store at 4°C for up to 7 days, or at -80°C for several weeks. Note that stability varies by sequence.

3. Stability Indicators:

• Cloudiness or precipitation may indicate degradation or improper solvent choice.

• Regular HPLC testing is advised for critical applications.

Our technical team provides customized storage recommendations for bulk orders. For specific queries, download our detailed peptide handling manual or contact support@hongkebiotech.com.